The 20 mg/kg fisetin dose is the most commonly referenced human extrapolation from the Yousefzadeh (2018) study. For a 70 kg adult, this equals approximately 1,400 mg of fisetin taken over 2 consecutive days. The protocol is repeated monthly or quarterly depending on individual goals and tolerance.

Can I take all 1,400 mg of fisetin at once?

Split dosing is preferred. Taking the full dose at once leads to a single plasma peak followed by rapid clearance (fisetin half-life: ~3 hours). Splitting into 2–3 doses throughout the day sustains therapeutic plasma levels longer.

What if I miss one of the two protocol days?

Continue with the remaining day. Missing one day is not catastrophic — partial senolysis still occurs. Make up the missed day within a reasonable window (within 48 hours) if possible.