Clinical senolytic trials use a combination of tissue biomarkers (p16, p21, SA-β-Gal from biopsies) and circulating biomarkers (IL-6, MMP-3, MMP-9, GDF15 from blood) to assess treatment efficacy. For self-directed monitoring without biopsy access, circulating inflammatory markers (IL-6, hsCRP) and functional assessments (gait speed, grip strength) provide the most practical evidence of senolytic activity.

What's the most practical biomarker panel for monitoring my own senolytic protocol?

Start with: hsCRP, IL-6, and GDF15 from a commercial lab. These three capture different aspects of senescent cell burden at low cost. Measure before starting, after 3 months, and every 6 months ongoing.

Where can I get p16 mRNA testing?

Specialized companies including Life Length (telomere + senescence), TruDiagnostic (epigenetic age + inflammation), and some academic labs offer p16 blood testing. Standard commercial labs do not currently provide this.